A novel scaffold protein, TANC, possibly a rat homolog of Drosophila rolling pebbles (rols), forms a multiprotein complex with various postsynaptic density proteins

Eur J Neurosci. 2005 Jan;21(2):339-50. doi: 10.1111/j.1460-9568.2005.03856.x.

Abstract

We cloned from the rat brain a novel gene, tanc (GenBank Accession No. AB098072), which encoded a protein containing three tetratricopeptide repeats (TPRs), ten ankyrin repeats and a coiled-coil region, and is possibly a rat homolog of Drosophila rolling pebbles (rols). The tanc gene was expressed widely in the adult rat brain. Subcellular distribution, immunohistochemical study of the brain and immunocytochemical studies of cultured neuronal cells indicated the postsynaptic localization of TANC protein of 200 kDa. Pull-down experiments showed that TANC protein bound PSD-95, SAP97, and Homer via its C-terminal PDZ-binding motif, -ESNV, and fodrin via both its ankyrin repeats and the TPRs together with the coiled-coil domain. TANC also bound the alpha subunit of Ca2+/calmodulin-dependent protein kinase II. An immunoprecipitation study showed TANC association with various postsynaptic proteins, including guanylate kinase-associated protein (GKAP), alpha-internexin, and N-methyl-D-aspartate (NMDA)-type glutamate receptor 2B and AMPA-type glutamate receptor (GluR1) subunits. These results suggest that TANC protein may work as a postsynaptic scaffold component by forming a multiprotein complex with various postsynaptic density proteins.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Amino Acid Sequence
  • Animals
  • Animals, Newborn
  • Ankyrin Repeat / genetics
  • Blotting, Northern
  • Blotting, Western / methods
  • Brain / cytology
  • Brain / metabolism
  • Cells, Cultured
  • Cloning, Molecular / methods
  • Crotalid Venoms / genetics
  • Crotalid Venoms / metabolism
  • Disks Large Homolog 4 Protein
  • Drosophila
  • Drosophila Proteins / chemistry*
  • Drosophila Proteins / genetics
  • Gene Expression Regulation / physiology
  • Humans
  • Immunohistochemistry / methods
  • Immunoprecipitation / methods
  • In Situ Hybridization / methods
  • Intracellular Signaling Peptides and Proteins
  • Lectins, C-Type / genetics
  • Lectins, C-Type / metabolism
  • Membrane Proteins / chemistry*
  • Membrane Proteins / genetics
  • Molecular Sequence Data
  • Multiprotein Complexes / metabolism*
  • Muscle Proteins / chemistry*
  • Muscle Proteins / genetics
  • Nerve Tissue Proteins / chemistry
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • Neurons / metabolism*
  • Protein Binding / physiology
  • Protein Structure, Tertiary
  • RNA, Messenger / biosynthesis
  • Rats
  • Receptors, AMPA / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Subcellular Fractions / metabolism

Substances

  • Crotalid Venoms
  • Disks Large Homolog 4 Protein
  • Dlg4 protein, rat
  • Drosophila Proteins
  • Intracellular Signaling Peptides and Proteins
  • Lectins, C-Type
  • Membrane Proteins
  • Multiprotein Complexes
  • Muscle Proteins
  • Nerve Tissue Proteins
  • RNA, Messenger
  • Receptors, AMPA
  • TANC1 protein, rat
  • postsynaptic density proteins
  • rols protein, Drosophila
  • glutamate receptor ionotropic, AMPA 1

Associated data

  • GENBANK/AB098072